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??Set yourself up for success with recombinant antibodies.
Recombinant antibody production occurs in vitro by cloning antibody genes for immune-specific heavy and light antibody chains into high-yield expression vectors. These vectors are then introduced into expression hosts (eg bacteria, yeast, or mammalian) to generate recombinant antibodies. Recombinant antibodies can be used wherever you would normally use a traditional monoclonal antibody.
In comparison, monoclonal antibodies are typically made using B-cells from an immunized animal to form immortal hybridoma cells that secrete the desired antibody clone. While this technique produces highly consistent, specific, and sensitive monoclonal antibodies in large quantities, over time hybridoma cell lines can experience genetic drift, resulting in slight variations to the antibodies produced. Antibodies against difficult targets, ie toxins, nucleotides, and membrane-bound proteins, can’t always be made with this in vivo model either.
Recombinant antibodies overcome the limitations of traditional antibody production to give you the highest level of consistency between batches, unrivaled reproducibility, confirmed specificity, and a guaranteed long-term supply.
?Because recombinant antibodies are developed from a unique set of genes, recombinant antibody production is controlled and reliable. Several problems with hybridoma production can be avoided, such as gene loss, gene mutations, and cell-line drift. This leads to antibodies with very high batch-to-batch consistency, giving you the highly reproducible results your research or drug development research requires. ?
?With recombinant technology, it is easier to improve antibody sensitivity through antibody engineering. The selection process for the desired clone occurs at both the hybridoma and recombinant cloning stages, allowing us to select the most favorable antibody qualities.
To ensure specificity, we use extensive validation methods, including knockout validation, to give you confidence in your results. The following examples demonstrate the differences in specificity between recombinant antibodies and other antibody types because the expected molecular weight band disappears in the knockout sample:
Figure 2. Our recombinant anti-TLE 1 antibody [EPR9386(2)] (left) being tested on knockout and wild-type samples against a monoclonal anti-TLE 1 [OTI1F5] antibody.
??Figure 3. Our recombinant anti-GRIM19 [EPR4471(2)] antibody (left) being tested on knockout and wild-type samples against our monoclonal anti-GRIM19 [6E1BH7]
High affinity, consistent between different batches
KD value is a quantitative measure of antibody affinity. The lower the Kd value, the higher the affinity of the antibody. On average, our recombinant RabMab antibodies have KD values in the picomolar (10-10–10-12) range, which is considered to be very high affinity (see the data for 863 RabMAb antibodies).
High-affinity antibodies allow greater sensitivity in assays, as they bind strongly to the antigen and maintain this bond better under difficult conditions compared to low-affinity antibodies. Analysis of KD values of our recombinant anti-PDL1 antibody shows that its affinity remains high across 5 different batches (Table 1).
?With the antibody genes isolated and the sequence known, antibody expression can be carried out at any scale and the long-term supply of antibody is guaranteed. This makes recombinant antibodies a great solution for long-term studies or using the same antibody across multiple samples.
Once the antibody-producing genes are isolated, animal-free in vitro production can be implemented. For antibodies generated using our phage display technology, even the gene of the antibody can be isolated with an animal-free procedure.
Our recombinant antibodies are featured in over 50,000 publications.
"In long-term studies or at service labs such as mine, we often use the same antibody for many samples. It is very important to have batch-to-batch consistency. I always test my protocols when receiving a new batch of antibody. One of the things I like about Abcam's RabMAb?s is that they always work the same, therefore almost no optimization is needed."
"All of the recombinant antibodies gave me a specific and nice band compared to the polyclonal antibody. It was consistent in terms of quality and experimental outcomes with only a low amount of protein required."
?“Previously we have tried several anti-H3K27M antibodies, but sometimes it was difficult to distinguish H3K27M-positive cells and non-specific signals. This antibody (H3K27M RabMAb?) showed a more specific and sensitive detection of the H3K27M protein with a very low background.”